Phospholamban is a type II membrane protein located in the sarcoplasmic reticulum (SR) of cardiac myocytes, smooth muscle myocytes and slow-twitch skeletal muscle myocytes. It is a regulator of Ca2+-uptake by the sarcoplasmic reticulum, and achieves this by inhibition of the Ca2+-pump, SERCA2. Phosphorlyation of particular residues in phospholamban blocks this inhibition of SERCA2, and thereby enhances Ca2+-uptake by the SR. This is critical to fight or flight stress responses. Over inhibition of SERCA function occurs in heart failure, and research to modify phospholamban function is being examined to find new ways to manage heart failure.
The scientific team at Badrilla were the first to produce phospho-specific antibodies to phospholamban (Drago & Colyer (1994) J. Biol. Chem. 269, 25073–25077). These have become the gold-standards for phospholamban research and have been used in >200 papers in the 2011& 2012. We now have antibodies to three phosphorylated forms of phospholamban (PLB, PLN): phospho-Serine10 (PKC site), phospho-Ser16 (PKA, PKG site) and phospho-Threonine17 (CamKII, Akt site), and to the total protein (mAb A1). These antibodies are available as anti-sera and as protein A affinity purified immunoglobulns. They are shipped in dry format (using a proprietary stabilizer formulation where necessary) for maximum product stability and to minimise our impact on the environment.
List Grid
6 Product(s)
-
anti-Phospholamban A1 Antibody (A010-14)
£193.59Description: Mouse monoclonal antibody to phospholamban.
Unit Volume: 20 μg
Tested Applications: Western blot (1:5000 dilution), IHC Microscopy (1:200 dilution), Ca2+ pump assay (1:500 dilution)
Antibody form: Lyophilised Affinity Purified IgG.
Species recognition: All mammalian species
Learn More -
anti-Phospholamban Phospho Serine-10 Affinity Purified Antibody (A010-10AP)
£193.59Description: Affinity purified Rabbit polyclonal antibody to phospholamban Phospho Ser-10
Unit volume: 50 µl
Tested Applications: Western blot (1:1000 dilution)
Antibody Form: IgG. Antibody Purity: Protein A affinity purified
Species Reactivity: Reacts with Phospho Ser-10 of phospholamban from most species (see alignment)
Learn More -
anti-Phospholamban Phospho Serine-16 (A010-12)
£193.59Description: Rabbit polyclonal antibody to phospholamban phospho Serine-16.
Unit volume: 20 µl
Tested Applications: Western blot (1:5000 dilution)
Antibody Form: IgG
Antibody Purity: Raw anti-serum
Species Reactivity: Reacts with Phospho Ser-16 of phospholamban from Human, mouse, rat, sheep, chicken, bovine, canine, ferret, hamster
Learn More -
anti-Phospholamban Phospho Serine-16 (A010-12AP)
£324.84Description: Affinity purified Rabbit polyclonal antibody to phospholamban Phospho Ser-16
Unit volume: 50 µl
Tested Applications: Western blot (1:5000 dilution), IHC Microscopy (1:200 dilution)
Antibody Form: IgG. Antibody Purity: Protein A affinity purified
Species Reactivity: Reacts with Phospho Ser-16 of phospholamban from bovine, canine, ferret, hamster, human, rat and sheep
Learn More -
anti-Phospholamban Phospho Threonine-17 anti-serum (A010-13)
£193.59Description: Rabbit Polyclonal Anti-Serum specific to Thr-17 phosphorylated forms of Phospholamban.
Unit Volume: 20 μl
Tested Applications: Western Blot (1:5000 dilution), IHC Microscopy (1:200 dilution)
Antibody Form: Lyophilised anti-serum
Species Recognition: All mammalian species, when phosphorylated on Thr-17.
Learn More -
anti-Phospholamban Phospho Threonine-17 (A010-13AP)
£324.84Description: Affinity Purified Rabbit Polyclonal antibody specific to Thr-17 phosphorylated forms of Phospholamban.
Unit Volume: 100 μl
Tested Applications: Western Blot (1:5000 dilution)
Antibody Form: Lyophilised Affinity Purified IgG.
Species Recognition: All mammalian species, when phosphorylated on Thr-17.
Learn More
List Grid
6 Product(s)