Description: A fully validated, highly specific, affinity purified mouse monoclonal antibody to phospholamban (anti-PLB | anti-PLN)
Background: The majority of calcium required for muscle contraction is mobilised from the Sarcoplasmic Reticulum (SR), to which it has to return to in order to facilitate relaxation. The (Ca2+- Mg2+) ATPase enzyme is responsible for the sequestration of calcium after its release from the SR, and is controlled by another sarcoplasmic reticulum protein called Phospholamban (PLB) in cardiac, smooth and slow-twitch skeletal muscle. The monoclonal antibody (A1) was raised against bovine cardiac PLB and recognises an epitope comprising of residues 7LTRSAIRRAS16. The antibody reacts with all forms of phospholamban PLB: monomer, oligomers, phosphorylated and non-phosphorylated. The antibody stimulates Ca2+ pump activity upon binding to PLB, to an extent equivalent to stoichiometric phosphorylation of PLB Manipulation of SERCA2:PLB ratios is a therapeutic strategy for heart failure. Elimination of PLB boosts SR Ca2+-load which can exacerbate pathological outcomes (apoptosis, arrhythmias) when combined with other alterations in SR function.
Unit Volume: 20 μg
Tested Applications: Western blot (1:5000 dilution), IHC Microscopy (1:200 dilution), Ca2+ pump assays (1:500 dilution)
Antibody form: Lyophilised Affinity Purified IgG.
Species recognition: All mammalian species
Full Publication list: 45 papers
Selected Publications:
SUMO1-dependent modulation of SERCA2a in heart failure.
W.J., and Hajjar, R.J. (2011). . Nature 477, 601-605.
Carbon monoxide exposure enhances arrhythmia after cardiac stress: involvement of oxidative stress.
André, L., Gouzi, F., Thireau, J., Meyer, G., Boissiere, J., Delage, M., Abdellaoui, A., Feillet-Coudray, C., Fouret, G., Cristol, J.P., et al. (2011). Basic Res Cardiol 106, 1235-1246.
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