SERCA2a is a Ca2+ pump located in the sarcoplasmic reticulum of cardiac and slow-twitch skeletal muscle. The enzyme transports Ca2+ from the cytoplasm into the SR using the hydrolysis of ATP as the energy source for this reaction. Two Ca2+ are transported for each ATP hydrolysed. This Ca2+ transport is the principal means of muscle relaxation in cardiac and slow-twitch skeletal muscle. The activity of SERCA2a is moderated by its interaction with phospholamban. SERCA2a is one isoform of SERCA2: this isoform differs from SERCA2b and SERCA2c at the extreme carboxy terminus only. Antibody A010-20 recognises the C-terminal sequence which distinguishes SERCA2a from SERCA2b and SERCA2c.
Tested applications: Western Blot (1:5000 dilution). Species recognition: The antibody reacts with SERCA2a from human, rat, mouse, rabbit, canine, and bovine species.
|Description||Lyophilised Rabbit polyclonal serum containing IgG antibody specific to SERCA2a|
|Immunogen||Synthetic peptide (C-LEPAILE997) corresponding to amino acids at the extreme C-terminus of SERCA2a|
|Species reactivity||The antibody recognises SERCA2a and binding is blocked in the presence of a peptide containing the epitope. The antibody reacts with SERCA2a from human, rat, mouse, rabbit, canine, and bovine species.|
|Tested applications||WB, ELISA and IHC microscopy|
|Recommended dilution||WB 1:5000|
|Optimization||Optimal dilutions to be determined by end user|
|Storage||Lyophilised antibody is stable at 4°C when stored with desiccant. Reconstitute lyophilised powder in 20 µl of 18 MΩ H2O, aliquot and store frozen at -80°C for 1 year. Avoid freeze - thaw cycles.|
|Storage||Lyophilised: 4 °C. Reconstituted:-80 °C|
|Datasheet||A010-20 Lot 0210-06
A010-20 Lot 0814-01
|Regulatory statement||Product for research use only. Not intended for diagnostic or therapeutic use.|