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    Calcineurin (pSer197) pAb serum

    $325.00

    In stock

    Product code: A010-80
    Unit Size: 100 μl
     

    A fully validated, highly specific rabbit polyclonal anti-serum to Serine-197 phosphorylated forms Calcineurin

    Cytoplasmic Ca2+ binds to calmodulin and activates the phosphatase calcineurin (Cn) a 60 kDa protein, and CaM kinase II (CaMKII). One action of Cn is to dephosphorylate cytoplasmic NFAT (nuclear factor of activated T cells), inducing its translocation to the nucleus where it activates antiapoptotic and hypertrophic target genes. The phosphorylation of Cn at Phospho Ser-197 site has been observed in cultured neonatal rat ventricular myocytes (NRVMs) over-expressing constitutively active CAMKII. This appears to inhibit Cn activity which in turn prevents the dephosphorylation of NFAT and prevents activation of NFAT regulated genes.

    Tested applications: Western blot (1:200 dilution). Species recognition: The antibody recognises Calcineurin in mouse/rat/human/monkey/other, when phosphorylated on Ser-197.

    Description Lyophilised rabbit polyclonal anti-serum containing IgG antibody specific for Ser-197 phosphorylated forms Calcineurin
    Immunogen Synthetic peptide CGKMARVFS(PO3H2)VLREES-amide corresponding to residues surrounding Ser at position 197 of Calcineurin
    Isotype IgG
    Purification Raw serum
    Species reactivity The antibody recognises Calcineurin in all mammalian species, when phosphorylated on Ser-197. Eptiope sequence also aligns with chicken and Xenopus
    Tested applications WB
    Recommended dilution WB: 1:200
    Optimization Optimal dilutions to be determined by end user
    Storage Lyophilised antibody is stable at 4°C when stored with desiccant. Reconstitute lyophilised powder in 100 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles.
    Storage Lyophilised: 4 °C. Reconstituted:-80 °C
    Datasheet A010-80

    MSDS A010-80

    Regulatory statement Product for research use only. Not intended for diagnostic or therapeutic use.

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    DISCLAIMER: Products for research use only (RUO) and are not intended for diagnostic or therapeutic use.

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