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    Calcineurin (pSer197) pAb IgG

    $325.00

    In stock

    Product code: A010-80AP
    Unit Size: 100 μl
     

    A fully validated, highly specific rabbit polyclonal antibody (IgG) to Serine-197 phosphorylated forms Calcineurin

    Cytoplasmic Ca2+ binds to calmodulin and activates the phosphatase calcineurin (Cn) a 60 kDa protein, and CaM kinase II (CaMKII). One action of Cn is to dephosphorylate cytoplasmic NFAT (nuclear factor of activated T cells), inducing its translocation to the nucleus where it activates antiapoptotic and hypertrophic target genes. The phosphorylation of Cn at Phospho Ser-197 site has been observed in cultured neonatal rat ventricular myocytes (NRVMs) over-expressing constitutively active CAMKII. This appears to inhibit Cn activity which in turn prevents the dephosphorylation of NFAT and prevents activation of NFAT regulated genes.

    Tested applications: Western blot (1:200 dilution). Species recognition: The antibody recognises calcineurin in mouse/rat/human/monkey/other, when phosphorylated on Ser-197.

    Description Lyophilised rabbit polyclonal IgG preparation containing antibodies specific for Ser-197 phosphorylated forms Calcineurin
    Immunogen Synthetic peptide CGKMARVFS(PO3H2)VLREES-amide corresponding to residues surrounding Ser at position 197 of Calcineurin
    Isotype IgG
    Purification IgG purified by affinity chromatography (protein A)
    Species reactivity The antibody recognises Calcineurin in all mammalian species, when phosphorylated on Ser-197. Eptiope sequence also aligns with chicken and Xenopus
    Tested applications WB
    Recommended dilution WB: 1:200
    Optimization Optimal dilutions to be determined by end user
    Storage Lyophilised antibody is stable at 4°C when stored with desiccant. Reconstitute lyophilised powder in 100 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles.
    Storage Lyophilised: 4 °C. Reconstituted:-80 °C
    Datasheet A010-80

    MSDS A010-80

    Regulatory statement Product for research use only. Not intended for diagnostic or therapeutic use.

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    DISCLAIMER: Products for research use only (RUO) and are not intended for diagnostic or therapeutic use.

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