Background: The L-type Ca2+ channel (LTCC, or Cav1.2 or dihydropyridine receptor) is a voltage gated Ca2+ channel of the plasma membrane in brain and heart, which is responsible for the influx of Ca2+ to initiate neurotransmitter release (in neurones) or muscle contraction (in cardiac muscle). The channel is controlled by a number of chemical influences including phosphorylation on Ser-1928 by PKA which enhances ion channel activity. Ser-1928 from rabbit, is conserved across all mammalian species and is thought to be phosphorylated in response to elevated cAMP in these species.
Tested applications: IHC Microscopy (1:100). Species recognition: all mammalian species, when phosphorylated on Ser-1928.
|Description||Lyophilised rabbit polyclonal anti-serum (A010-70) containing IgG antibody specific for Cav1.2 Phospho Ser-1928 (PKA site).|
|Immunogen||Synthetic peptide (LGRRAS(PO3H2)FHLE1932) corresponding to amino acids surrounding the phosphorylated serine residue at position 1928 of α1c subunit of Cav1.2 from rabbit conjugated to KLH.|
|Species reactivity||The epitope peptide aligns exactly to Human Cav1.2 α1c isoforms 1-23 (See website - Ep/Is alignment). The antibody has been shown to recognise phosphorylated serine-1928 of rat Cav1.2 (α1c), and binding is blocked in the presence of a peptide containing the Phospho Ser-1928 epitope.|
|Tested applications||IHC microscopy|
|Recommended dilution||IHC microscopy: 1:100|
|Optimization||Optimal dilutions to be determined by end user|
|Storage||Lyophilised antibody is stable at 4°C when stored with desiccant. Reconstitute lyophilised powder in 50 µl of 18 MΩ H2O, aliquot and store frozen at -80°C for 1 year. Avoid freeze - thaw cycles.|
|Storage||Lyophilised: 4 °C. Reconstituted:-80 °C|
|Regulatory statement||Product for research use only. Not intended for diagnostic or therapeutic use.|