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    mEOS2 pAb

    $325.00

    In stock

    Product code: A010-mEOS2
    Unit Size: 50 μg 

    Lyophilised rabbit polyclonal protein A affinity purified antibody

    GFP was introduced as a tool for molecular biology over 20 years ago and is thought of as one of those most significant contributions to modern microscopy. Many related fluorescent proteins are subject to oligomerisation and photobleaching and as such, limit the resolution obtainable from their use. mEOS2 is a photoactivatable green to red photoconvertible fluorescent protein derived from Anthozoa. It has recently emerged and is set to make significant contributions to the enhancement of super resolution microscopy for the study of cellular ultra structure and protein trafficking. There are already a number of papers describing its use in the developing field of Photoactivated localisation microscopy (PALM) reversible saturable optical fluorescence transitions (RESOLFT), stochastic optical reconstruction microscopy (STORM) and three-dimensional high-resolution imaging (McKinney et al. 2009). A010-mEOS2 is a protein A affinity purified rabbit polyclonal antibody raised against purified mEOS2 expressed in E.coli and stains mEOS2 fusion proteins expressed in bacteria and mammalian cells in both western blotting and immunofluorescence microscopy applications.

    Description Lyophilised rabbit polyclonal protein A affinity purified antibody
    Immunogen Purified mEOS2 expressed as a GST fusion protein in BL21 Rosetta E.coli cells
    Isotype IgG
    Purification Protein A affinity purified
    Species reactivity N/A
    Tested applications WB
    Recommended dilution WB 1:10,000 for high expression, 1:1000 for low level expression
    Optimization Optimal dilutions to be determined by end user
    Storage Lyophilised antibody is stable at 4 °C when stored with desiccant. Reconstitute lyophilised powder in 50 µl of 18 MΩ H2O for a 1mg/ml solution with stable buffering conditions. Aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles.
    Storage Lyophilised 4 °C Reconstituted -80 °C
    Datasheet A010-mEOS2

    MSDS A010-mEOS2

    Regulatory statement Product for research use only. Not intended for diagnostic or therapeutic use.

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    DISCLAIMER: Products for research use only (RUO) and are not intended for diagnostic or therapeutic use.

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