PDP3 peptide is an activator of protein phosphatase-1 enzyme. It is fused to a polybasic peptide which renders the peptide cell permeable. The peptide interferes with the binding of regulatory (inhibitory) proteins to PP-1 and liberates a PDP3-PP1 catalytically active enzyme (EC50 176 nM; 1). This reduces protein phosphorylation within cells and affects numerous cellular processes including Ca2+-signaling via IP3R (1). It provokes Ca2+-oscillations in HeLa cells (1). It does not affect PP-2A or PP-2B.
PDP3 is long lived inside cells. It contains the unnatural amino acid Bpa (L-4-Benzoylphenylalanine) located C-terminally to the RVTF-type docking motif, which makes it exceptionally stable inside cells. It induces long-lasting activation of PP-1 which extends beyond the washout of the peptide from a cell perfusion experiment (1).
PDP3 in this current form is cell permeable.
1: Reither, G., Chatterjee, J., Beullens, M., Bollen, M., Schultz, C & Kohn, M (2013) Chemical activators of protein phosphatase-1 induce calcium release inside intact cells. Chemistry & Biology 20, 1179-86
PDP3 Peptide, cell permeable
Acetyl group (blocking N-terminus)
Amide (blocking carboxy terminus)
|Counter ions||Trifluroacetate (TFA)|
Analysis carried out using a 100Å 4.6 x 150mm XB-C18 column, gradient from 0%-80% acetonitrile, in 15 minutes. 60 degC. Purity determined as 98.8%
Molecular ion predicted: 3016.57. Observed: 3016.5 [M+3H]
Store as supplied at -20°C for up to 1 year. Store desiccated. Make fresh solutions prior to use.
The product was purified in acetonitrile and water containing 0.1% trifluoroacetic acid (TFA) prior to lyophilisation. The product will therefore be present as its trifluoroacetic acid salt.
|Regulatory statement||Product for research use only. Not intended for diagnostic or therapeutic use.|