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    PDP3 peptide, cell permeable

    RRP: $190.18

    Special Price $150.00

    In stock

    Product code: P010-610
    Unit Size: 1 mg peptide

    Ac-RRKRPKRKRKNARVTF-Bpa-EAAEII-NH2

    PDP3 peptide is an activator of protein phosphatase-1 enzyme. It is fused to a polybasic peptide which renders the peptide cell permeable. The peptide interferes with the binding of regulatory (inhibitory) proteins to PP-1 and liberates a PDP3-PP1 catalytically active enzyme (EC50 176 nM; 1). This reduces protein phosphorylation within cells and affects numerous cellular processes including Ca2+-signaling via IP3R (1). It provokes Ca2+-oscillations in HeLa cells (1). It does not affect PP-2A or PP-2B.

    PDP3 is long lived inside cells. It contains the unnatural amino acid Bpa (L-4-Benzoylphenylalanine) located C-terminally to the RVTF-type docking motif, which makes it exceptionally stable inside cells. It induces long-lasting activation of PP-1 which extends beyond the washout of the peptide from a cell perfusion experiment (1).

    PDP3 in this current form is cell permeable.

    1: Reither, G., Chatterjee, J., Beullens, M., Bollen, M., Schultz, C & Kohn, M (2013) Chemical activators of protein phosphatase-1 induce calcium release inside intact cells. Chemistry & Biology 20, 1179-86

    Peptide name

    PDP3 Peptide, cell permeable

    Peptide sequence

    Ac-RRKRPKRKRKNARVTF-Bpa-EAAEII-NH2

    N-terminus

    Acetyl group (blocking N-terminus)

    C-terminus

    Amide (blocking carboxy terminus)

    Counter ions Trifluroacetate (TFA)
    Molecular weight

    3016.6

    HPLC analysis

    Analysis carried out using a 100Å 4.6 x 150mm XB-C18 column, gradient from 0%-80% acetonitrile, in 15 minutes. 60 degC. Purity determined as 98.8%

    MS analysis

    Molecular ion predicted: 3016.57. Observed: 3016.5 [M+3H]

    Appearance

    off-white solid

    Solubilisation

    DMSO

    Storage

    Store as supplied at -20°C for up to 1 year. Store desiccated. Make fresh solutions prior to use.

    Datasheet

    P010-610

     

    MSDS P010-610

    Notes

    The product was purified in acetonitrile and water containing 0.1% trifluoroacetic acid (TFA) prior to lyophilisation. The product will therefore be present as its trifluoroacetic acid salt.

    Regulatory statement Product for research use only. Not intended for diagnostic or therapeutic use.

    Contact Us

    Badrilla Ltd.

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    Leeds LS2 8AJ

    United Kingdom

     

    +44 (0)7801 730618

     

    info@badrilla.com

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    DISCLAIMER: Products for research use only (RUO) and are not intended for diagnostic or therapeutic use.

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