Badrilla’s Click S-Palmitoylation Detection⁷⁷⁵ Kit^TM provides a quick, convenient and robust method to identify S-palmitoylation of heterologously expressed proteins in cultured cells. The assay is based on the copper catalysed click chemistry reaction and facilitates, with four simple steps – Label, Lyse, Click and Detect - determination of protein palmitoylation.

Click S-Palmitoylation Detection⁷⁷⁵  Kit Enables

•           Detection of S-palmitoylated proteins in cultured cells.

•           Processing of 4 x 24 well cell culture plates (i.e. 96 samples in total).

•           Convenient stopping point after labelling treatment.

• A modified ‘biotin-switch’ assay
• Labels all S-nitrosylated sites in sample ('omics')
• DETECT S-NO proteins in whole cells, tissues or lysates
• Colourimetric or fluorimetric detection
• Sufficient reagents for 3 x 20 samples

DPc10 inactive peptide is a 36-residue peptide that differs from DPc10 (Gly2460-Pro2495, rabbit) at one position (R2475S) and represents the sequence of a known CPVT mutant RYR2 domain. This peptide binds weakly or fails to bind to the DPc10 interface and does NOT affect channel function. It serves as a negative control for research projects using DPc10 peptide.  

Pack size 200µg.

DPc10 peptide is a 36-residue peptide corresponding to the Gly2460-Pro2495 region of rabbit RyR2. It interacts with RYR2 and provokes abnormal channel activity usually seen in mutant RYR2 channels associated with CPVT. Elevates Ca2+-leak, increases Ca2+-spark activity, is pro-arrhythmic, and provokes hypertrophy in cardiac myocytes.  

Pack size 200µg.

Convenient, cost effective sampler pack containing four DPc10 peptide derivatives (200µg each) as follows:

1. DPc10 Peptide (Cat. A010-514)
2. DPc10 Inactive Peptide (Cat. A010-515)
3. Fluorescein DPc10 Peptide (Cat. A010-516)
4. Fluorescein DPc10 Inactive Peptide (Cat. A010-517)

 Affinity purified Rabbit polyclonal antibody to Erk1/2 phospho threonine 188

• Tested applications: Western blot (1:1000 dilution)
• Protein A affinity purified
• Species Reactivity: peptide aligns with sequence from both Mouse and Human. Threonine 188 is number in relation to the mouse sequence of ERK2.
• Unit size: 50 µl / µg

Fluorescein derivative of the DPc10 inactive peptide, a 36-residue peptide that differs from DPc10 (Gly2460-Pro2495, rabbit) at one position (R2475S) and represents the sequence of a known CPVT mutant RYR2 domain. This peptide binds weakly or fails to bind to the DPc10 interface and does NOT affect channel function. It serves as a negative control for research projects using DPc10 peptide.

Pack size 200µg.

Fluorescein derivative of DPc10 peptide, a 36-residue peptide corresponding to the Gly2460-Pro2495 region of rabbit RyR2. It interacts with RYR2 and provokes abnormal channel activity usually seen in mutant RYR2 channels associated with CPVT.  Elevates Ca2+-leak, increases Ca2+-spark activity, is pro-arrhythmic, and provokes hypertrophy in cardiac myocytes.

Pack size 200µg

A fully validated, highly specific, affinity purified rabbit polyclonal antibody to GFP, EGFP, PA-GFP, CFP and YFP

• Suitable Western blot (1:10,000 dilution), IF Microscopy (1:500 - 5000 dilution)
• Lyophilised affinity purified IgG
• Unit size: 5 μg - enough for 31 blots