The Badrilla CAPTUREome^TM S-Palmitoylated protein kit is a simple and easy way of determining whether your protein of interest is post translationally modified with a palmitate group. The assay is based on the acyl-RAC (resin assisted capture) method and facilitates, with four simple steps – Block, Cleave, Capture and Analysis -  determination of protein palmitoylation.

Kit highlights

• Enables capture of S-palmitoylated proteins.
• Kit designed to process 4 x 5 biological samples with paired negative controls (i.e. 40 samples in total).
• Convenient stopping point after blocking treatment.
• Assay suitable for downstream SDS-PAGE, western blot or mass spectrometry analysis.

The new CAPTUREome^TM S-Palmitoylation Mini Kit from Badrilla represents an affordable gateway into the emerging field of palmitoylation research. The Mini Kit, based on acyl RAC (resin assisted capture) technology, enables rapid determination of protein palmitoylation status from as little as 1mg of total cell lysate protein.

• Competitively priced entry level kit.
• Enables quick and easy capture of S-palmitoylated proteins from as little as 1mg of total cell lysate protein.
• Kit designed to process 2 x 3 biological samples with paired negative controls (i.e. 12 samples in total).
• Convenient stopping point after blocking treatment.
• Assay suitable for downstream SDS-PAGE, western blot or mass spectrometry analysis.

Dantrolene inhibits release of Ca²⁺ from the sarcoplasmic reticulum (SR) via inhibition of ryanodine receptor (RYR) channels. Learn More

• A modified ‘biotin-switch’ assay
• Labels all S-nitrosylated sites in sample ('omics')
• DETECT S-NO proteins in whole cells, tissues or lysates
• Colourimetric or fluorimetric detection
• Sufficient reagents for 3 x 20 samples

DPc10 inactive peptide is a 36-residue peptide that differs from DPc10 (Gly2460-Pro2495, rabbit) at one position (R2475S) and represents the sequence of a known CPVT mutant RYR2 domain. This peptide binds weakly or fails to bind to the DPc10 interface and does NOT affect channel function. It serves as a negative control for research projects using DPc10 peptide.  

Pack size 200µg.

DPc10 peptide is a 36-residue peptide corresponding to the Gly2460-Pro2495 region of rabbit RyR2. It interacts with RYR2 and provokes abnormal channel activity usually seen in mutant RYR2 channels associated with CPVT. Elevates Ca2+-leak, increases Ca2+-spark activity, is pro-arrhythmic, and provokes hypertrophy in cardiac myocytes.  

Pack size 200µg.

Convenient, cost effective sampler pack containing four DPc10 peptide derivatives (200µg each) as follows:

1. DPc10 Peptide (Cat. A010-514)
2. DPc10 Inactive Peptide (Cat. A010-515)
3. Fluorescein DPc10 Peptide (Cat. A010-516)
4. Fluorescein DPc10 Inactive Peptide (Cat. A010-517)

Proteasome-specific and highly potent, cell permeable, irreversible inhibitor. Reacts with all three active sites of the proteasome, primarily targets chymotrypsin-like activity.

 Affinity purified Rabbit polyclonal antibody to Erk1/2 phospho threonine 188

• Tested applications: Western blot (1:1000 dilution)
• Protein A affinity purified
• Species Reactivity: peptide aligns with sequence from both Mouse and Human. Threonine 188 is number in relation to the mouse sequence of ERK2.
• Unit size: 50 µl / µg