Badrilla’s Click S-Palmitoylation Detection⁷⁷⁵ Kit^TM provides a quick, convenient and robust method to identify S-palmitoylation of heterologously expressed proteins in cultured cells. The assay is based on the copper catalysed click chemistry reaction and facilitates, with four simple steps – Label, Lyse, Click and Detect - determination of protein palmitoylation.

Click S-Palmitoylation Detection⁷⁷⁵  Kit Enables

•           Detection of S-palmitoylated proteins in cultured cells.

•           Processing of 4 x 24 well cell culture plates (i.e. 96 samples in total).

•           Convenient stopping point after labelling treatment.

• A modified ‘biotin-switch’ assay
• Labels all S-nitrosylated sites in sample ('omics')
• DETECT S-NO proteins in whole cells, tissues or lysates
• Colourimetric or fluorimetric detection
• Sufficient reagents for 3 x 20 samples

The new Badrilla SiteCounter^TM S-Palmitoylated protein kit is a quick and easy way to quantify the extent of S-palmitoylation of your protein of interest. Using a straightforward mass tag approach, modification of each cysteine residue results in an incremental increase in the mass of the protein.  The number of mobility shifts (as detected by western blot analysis) is observed as a ladder of protein bands, each ‘step’ in the ladder representing an additional s-palmitoylated cysteine residue.

Kit highlights

• Enables determination of the number of S-palmitoylation sites on a protein
• Enables calculation of S-palmitoylation stoichiometry
• Kit designed to process 2 x 6 biological samples with paired negative controls (i.e. 24 samples in total).
• Convenient stopping point after blocking treatment.
• Assay suitable for downstream western blot analysis