The Badrilla CAPTUREome^TM S-Palmitoylated protein kit is a simple and easy way of determining whether your protein of interest is post translationally modified with a palmitate group. The assay is based on the acyl-RAC (resin assisted capture) method and facilitates, with four simple steps – Block, Cleave, Capture and Analysis -  determination of protein palmitoylation.

Kit highlights

• Enables capture of S-palmitoylated proteins.
• Kit designed to process 4 x 5 biological samples with paired negative controls (i.e. 40 samples in total).
• Convenient stopping point after blocking treatment.
• Assay suitable for downstream SDS-PAGE, western blot or mass spectrometry analysis.

• A modified ‘biotin-switch’ assay
• Labels all S-nitrosylated sites in sample ('omics')
• DETECT S-NO proteins in whole cells, tissues or lysates
• Colourimetric or fluorimetric detection
• Sufficient reagents for 3 x 20 samples

Affinity purified mouse monoclonal antibody clone 2E11 to nitrotyrosine

• Suitable for use in Western blot (1:2000 dilution)
• Antibody Form: IgG2b
• Protein A affinity purified
• Reacts with Nitrotyrosine either free or incorporated in proteins such as human serum nitro albumin, nitro haemoglobin and nitro insulin. All species
• Unit size: 50 µl

 Affinity purified Mouse monoclonal antibody clone 6B2 to NitroTyrosine (NY)

• Suitable for use in Western blot (1:5000 dilution)
• Protein A affinity purified
• Reacts with Nitrotyrosine either free or incorporated in proteins such as human serum nitro albumin, nitro haemoglobin and nitro insulin. All species
• Unit Size: 20 µl

A convenient reagent pack containing a high integrity nitrotyrosine monoclonal antibody and associated positive and negative controls.

The new Badrilla SiteCounter^TM S-Palmitoylated protein kit is a quick and easy way to quantify the extent of S-palmitoylation of your protein of interest. Using a straightforward mass tag approach, modification of each cysteine residue results in an incremental increase in the mass of the protein.  The number of mobility shifts (as detected by western blot analysis) is observed as a ladder of protein bands, each ‘step’ in the ladder representing an additional s-palmitoylated cysteine residue.

Kit highlights

• Enables determination of the number of S-palmitoylation sites on a protein
• Enables calculation of S-palmitoylation stoichiometry
• Kit designed to process 2 x 6 biological samples with paired negative controls (i.e. 24 samples in total).
• Convenient stopping point after blocking treatment.
• Assay suitable for downstream western blot analysis