The post-translational modification of individual residues in a protein is an important feature of protein regulation in health and disease. Highly specific monoclonal antibodies to chemically modified amino acids have been assembled. All of these antibodies have been carefully validated for use in western blotting.
Badrilla’s Click S-Palmitoylation Detection775 KitTM provides a quick, convenient and robust method to identify S-palmitoylation of heterologously expressed proteins in cultured cells. The assay is based on the copper catalysed click chemistry reaction and facilitates, with four simple steps – Label, Lyse, Click and Detect - determination of protein palmitoylation.
Click S-Palmitoylation Detection775 Kit Enables
• Detection of S-palmitoylated proteins in cultured cells.
• Processing of 4 x 24 well cell culture plates (i.e. 96 samples in total).
• Convenient stopping point after labelling treatment.Learn More
The new Badrilla SiteCounterTM S-Palmitoylated protein kit is a quick and easy way to quantify the extent of S-palmitoylation of your protein of interest. Using a straightforward mass tag approach, modification of each cysteine residue results in an incremental increase in the mass of the protein. The number of mobility shifts (as detected by western blot analysis) is observed as a ladder of protein bands, each ‘step’ in the ladder representing an additional s-palmitoylated cysteine residue.
The new Badrilla SiteCounterTM S-Palmitoylated Protein Mini Kit is a quick and easy way to quantify S-palmitoylation. Competitively priced, this starter kit provides a gateway into determining the number of palmitoylation sites in your protein of interest
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