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    S-Palmitoylation Tools

    S-Palmitoylation Tools

    Palmitoylation has emerged as an important post translational modification regulating protein function, intracellular trafficking and protein-protein as well as protein-lipid interactions. In particular it has generated much interest in the cardiovascular research community since it has been shown to regulate the function of key proteins that contribute to normal Ca2+ cycling and therefore myocyte contraction, as well as G-protein coupled receptors, important modulators of myocardial contractility. 

    Palmitoylation is the covalent attachment of a 16-carbon saturated fatty acid to a cysteine residue within a protein through the formation of a thioester or S-acyl bond.  The process is mediated by a set of enzymes referred to as palmitoyl acyl transferases (PATs) together with the fatty acid donor palmitoyl CoA. There are 24 PAT enzymes which are characterised by a canonical zinc binding motif Asp-His-His-Cys (zDHHC). Furthermore, over 8000 palmitoylated proteins have been identified (a database of these proteins can be found at http://swisspalm.epfl.ch/ ). Palmitoylation can be constitutive in some instances, but can also be dynamic and reversible, in a manner analogous to phosphorylation – the palmitate group being removed by the activity of acyl palmitoyl thioesterases (APTs).

    Learn more about palmitoylation from our knowledge resource : https://badrilla.com/project-landing/

    3 Item(s)

    1. Click S-Palmitoylation Detection-775 Kit™

      Product code: K010-410

      Badrilla’s Click S-Palmitoylation Detection775 KitTM provides a quick, convenient and robust method to identify S-palmitoylation of heterologously expressed proteins in cultured cells. The assay is based on the copper catalysed click chemistry reaction and facilitates, with four simple steps – Label, Lyse, Click and Detect - determination of protein palmitoylation.

      Click S-Palmitoylation Detection775  Kit Enables

      •           Detection of S-palmitoylated proteins in cultured cells.

      •           Processing of 4 x 24 well cell culture plates (i.e. 96 samples in total).

      •           Convenient stopping point after labelling treatment.

      Learn More
    2. SiteCounter™ S-Palmitoylated Protein Kit

      Product code: K010-312

      The new Badrilla SiteCounterTM S-Palmitoylated protein kit is a quick and easy way to quantify the extent of S-palmitoylation of your protein of interest. Using a straightforward mass tag approach, modification of each cysteine residue results in an incremental increase in the mass of the protein.  The number of mobility shifts (as detected by western blot analysis) is observed as a ladder of protein bands, each ‘step’ in the ladder representing an additional s-palmitoylated cysteine residue.

      Kit highlights

      • Enables determination of the number of S-palmitoylation sites on a protein
      • Enables calculation of S-palmitoylation stoichiometry
      • Kit designed to process 2 x 6 biological samples with paired negative controls (i.e. 24 samples in total).
      • Convenient stopping point after blocking treatment.
      • Assay suitable for downstream western blot analysis
      Learn More
    3. SiteCounter™ S-Palmitoylated Protein Mini Kit

      Product code: K010-313

      The new Badrilla SiteCounterTM S-Palmitoylated Protein Mini Kit is a quick and easy way to quantify S-palmitoylation. Competitively priced, this starter kit provides a gateway into determining the number of palmitoylation sites in your protein of interest

      Kit highlights

      • Competitively priced entry level kit.
      • Enables detection of the number S-palmitoylated sites within your protein of interest and calculation of palmitoylation stoichiometry.
      • Kit designed to process 2 x 2 biological samples with paired negative controls (i.e. 8 samples in total).
      • Convenient stopping point after blocking treatment.
      • Assay suitable for downstream western blot analysis
      Learn More

    3 Item(s)

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    DISCLAIMER: Products for research use only (RUO) and are not intended for diagnostic or therapeutic use.

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