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    S-Nitrosylation Tools

    S-Nitrosylation Tools

    Nitric oxide (NO) plays an important role in the regulation of cardiovascular function. In addition to the classic NO activation of the cGMP-dependent pathway, NO can also regulate cell function through protein S-nitrosylation, a redox dependent, thiol-based, reversible posttranslational protein modification that involves attachment of an NO moiety to a nucleophilic protein sulfhydryl group.

    A better understanding of the mechanisms regulating protein S-nitrosylation will provide us new therapeutic insights and targets for intervention in cardiovascular disease. Badrilla offers a growing range of antibodies and detection kits to support your nitric oxide and S-nitrosylaltion research projects; further details for each of these products can be found below.

    8 Item(s)

    1. CAPTUREome™ S-Palmitoylated Protein Kit

      $499.00
      Product code: K010-311

      The Badrilla CAPTUREomeTM S-Palmitoylated protein kit is a simple and easy way of determining whether your protein of interest is post translationally modified with a palmitate group. The assay is based on the acyl-RAC (resin assisted capture) method and facilitates, with four simple steps – Block, Cleave, Capture and Analysis -  determination of protein palmitoylation.

      Kit highlights

      • Enables capture of S-palmitoylated proteins.
      • Kit designed to process 4 x 5 biological samples with paired negative controls (i.e. 40 samples in total).
      • Convenient stopping point after blocking treatment.
      • Assay suitable for downstream SDS-PAGE, western blot or mass spectrometry analysis.
      Learn More
    2. CAPTUREome™ S-Palmitoylated Protein Mini Kit

      $148.51
      Product code: K010-310

      The new CAPTUREomeTM S-Palmitoylation Mini Kit from Badrilla represents an affordable gateway into the emerging field of palmitoylation research. The Mini Kit, based on acyl RAC (resin assisted capture) technology, enables rapid determination of protein palmitoylation status from as little as 1mg of total cell lysate protein.

      • Competitively priced entry level kit.
      • Enables quick and easy capture of S-palmitoylated proteins from as little as 1mg of total cell lysate protein.
      • Kit designed to process 2 x 3 biological samples with paired negative controls (i.e. 12 samples in total).
      • Convenient stopping point after blocking treatment.
      • Assay suitable for downstream SDS-PAGE, western blot or mass spectrometry analysis.
      Learn More
    3. DETECTome™ S-nitrosylated protein kit

      $368.00
      Product code: A010-105
      A convenient and easy-to-use kit incorporating a modification of the “biotin-switch” method to allow for the direct visualisation of S-nitrosylated proteins in whole cells or tissues, as well as by western blot analysis. 
       
      • A modified ‘biotin-switch’ assay
      • Labels all S-nitrosylated sites in sample ('omics')
      • DETECT S-NO proteins in whole cells, tissues or lysates
      • Colourimetric or fluorimetric detection
      • Sufficient reagents for 3 x 20 samples
      Learn More
    4. Nitrotyrosine mAb (clone 2E11)

      $325.00
      Product code: A010-2E11

      Affinity purified mouse monoclonal antibody clone 2E11 to nitrotyrosine

      • Suitable for use in Western blot (1:2000 dilution)
      • Antibody Form: IgG2b
      • Protein A affinity purified
      • Reacts with Nitrotyrosine either free or incorporated in proteins such as human serum nitro albumin, nitro haemoglobin and nitro insulin. All species
      • Unit size: 50 µl
      Learn More
    5. Nitrotyrosine mAb (Clone 6B2)

      $325.00
      Product code: A010-6B2AP

       Affinity purified Mouse monoclonal antibody clone 6B2 to NitroTyrosine (NY)

      • Suitable for use in Western blot (1:5000 dilution)
      • Protein A affinity purified
      • Reacts with Nitrotyrosine either free or incorporated in proteins such as human serum nitro albumin, nitro haemoglobin and nitro insulin. All species
      • Unit Size: 20 µl
      Learn More
    6. Nitrotyrosine Western blot starter pack

      $420.00
      Product code: A010-513

      A convenient reagent pack containing a high integrity nitrotyrosine monoclonal antibody and associated positive and negative controls.

      Learn More
    7. SiteCounter™ S-Palmitoylated Protein Kit

      $499.00
      Product code: K010-312

      The new Badrilla SiteCounterTM S-Palmitoylated protein kit is a quick and easy way to quantify the extent of S-palmitoylation of your protein of interest. Using a straightforward mass tag approach, modification of each cysteine residue results in an incremental increase in the mass of the protein.  The number of mobility shifts (as detected by western blot analysis) is observed as a ladder of protein bands, each ‘step’ in the ladder representing an additional s-palmitoylated cysteine residue.

      Kit highlights

      • Enables determination of the number of S-palmitoylation sites on a protein
      • Enables calculation of S-palmitoylation stoichiometry
      • Kit designed to process 2 x 6 biological samples with paired negative controls (i.e. 24 samples in total).
      • Convenient stopping point after blocking treatment.
      • Assay suitable for downstream western blot analysis
      Learn More
    8. SiteCounter™ S-Palmitoylated Protein Mini Kit

      $148.51
      Product code: K010-313

      The new Badrilla SiteCounterTM S-Palmitoylated Protein Mini Kit is a quick and easy way to quantify S-palmitoylation. Competitively priced, this starter kit provides a gateway into determining the number of palmitoylation sites in your protein of interest

      Kit highlights

      • Competitively priced entry level kit.
      • Enables detection of the number S-palmitoylated sites within your protein of interest and calculation of palmitoylation stoichiometry.
      • Kit designed to process 2 x 2 biological samples with paired negative controls (i.e. 8 samples in total).
      • Convenient stopping point after blocking treatment.
      • Assay suitable for downstream western blot analysis
      Learn More

    8 Item(s)

    Shop By
    Clonality
    1. mAb (3)
    Product type
    1. Antibody (3)
    2. Capture kit (2)
    3. Detection kit (3)
    Target
    1. Nitrotyrosine (3)
    2. S-NO (5)
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    +44 (0)7801 730618

    info@badrilla.com

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    DISCLAIMER: Products for research use only (RUO) and are not intended for diagnostic or therapeutic use.

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