Synthetic peptide (M281HRQET(PO3H2)VDC289) corresponding to amino acids surrounding the phosphorylated threonine residue at position 286 or 287 of CaMKII, which was conjugated to keyhole limpet hemocyanin (KLH) by carbodiimide cross linkage.
Reacts with rat. Not yet tested in other species. However, from primary sequence analysis, the antibody will recognise Phospho Thr-286 (or 287) in all isoforms (α, β, γ and δ) from human, rat and mouse. The antibody is also expected to react with various isoforms from chicken, cow, pig, rabbit, orangutan, zebrafish, ferret and Xenopus laevis.
Lyophilised antibody is stable at 4°C when stored with desiccant. Reconstitute lyophilised powder in 50 µl of 18 MΩ H2O, aliquot and store frozen at -80°C for 1 year. Avoid freeze – thaw cycles. Lyophilised: 4°C. Reconstituted:-80°C