SiteCounterTM S-Palmitoylated protein kit is a quick and easy way to quantify the extent of S-palmitoylation of your protein of interest from cell or tissue lysates: it will allow you to visualise the number of S-palmitoylation sites in a target protein. Kit sufficient for 2 experiments of 6 samples and corresponding (negative) controls. NB revised blocking step from Aug 2023: use correct protocol for your kit.
A chemistry kit to perform the 4 stages of palmitoylation site counting: Block, Cleave, Tag and Analyse – (by SDS-PAGE & western blotting). Each S-palmitoylated residue is labelled with a mass tag causing an electrophoretic shift in the apparent molecular weight of the target protein. Multiple S-palmitoylation sites result in multiple MW species of the target protein, which can be counted.