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Badrilla Ltd, founded in 2003, is an established life science company providing high quality products for the molecular cardiovascular researcher located within major pharmaceutical, biotech, and academic establishments worldwide... Read More

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Professor Colyer established Badrilla in 2003, combining substantive entrepeneurial experience with a deep understanding of the cardiovascular research field. The company has grown organically, reinvesting revenues into... Read More
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Residue Modification
S-Palmitoylation Tools

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S-Palmitoylation Tools

Palmitoylation has emerged as an important post translational modification regulating protein function, intracellular trafficking and protein-protein as well as protein-lipid interactions. In particular it has generated much interest in the cardiovascular research community since it has been shown to regulate the function of key proteins that contribute to normal Ca²⁺ cycling and therefore myocyte contraction, as well as G-protein coupled receptors, important modulators of myocardial contractility.

Palmitoylation is the covalent attachment of a 16-carbon saturated fatty acid to a cysteine residue within a protein through the formation of a thioester or S-acyl bond. The process is mediated by a set of enzymes referred to as palmitoyl acyl transferases (PATs) together with the fatty acid donor palmitoyl CoA. There are 24 PAT enzymes which are characterised by a canonical zinc binding motif Asp-His-His-Cys (zDHHC). Furthermore, over 8000 palmitoylated proteins have been identified (a database of these proteins can be found at http://swisspalm.epfl.ch/ ). Palmitoylation can be constitutive in some instances, but can also be dynamic and reversible, in a manner analogous to phosphorylation – the palmitate group being removed by the activity of acyl palmitoyl thioesterases (APTs).

CAPTUREome™ S-Palmitoylated Protein Kit

$499.00

Product code: K010-311
The Badrilla CAPTUREome^TM S-Palmitoylated protein kit is a simple and easy way of determining whether your protein of interest is post translationally modified with a palmitate group. The assay is based on the acyl-RAC (resin assisted capture) method and facilitates, with four simple steps – Block, Cleave, Capture and Analysis - determination of protein palmitoylation.

Kit highlights
- Enables capture of S-palmitoylated proteins.
- Kit designed to process 4 x 5 biological samples with paired negative controls (i.e. 40 samples in total).
- Convenient stopping point after blocking treatment.
- Assay suitable for downstream SDS-PAGE, western blot or mass spectrometry analysis.
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CAPTUREome™ S-Palmitoylated Protein Mini Kit

$199.00

Product code: K010-310
The new CAPTUREome^TM S-Palmitoylation Mini Kit from Badrilla represents an affordable gateway into the emerging field of palmitoylation research. The Mini Kit, based on acyl RAC (resin assisted capture) technology, enables rapid determination of protein palmitoylation status from as little as 1mg of total cell lysate protein.
- Competitively priced entry level kit.
- Enables quick and easy capture of S-palmitoylated proteins from as little as 1mg of total cell lysate protein.
- Kit designed to process 2 x 3 biological samples with paired negative controls (i.e. 12 samples in total).
- Convenient stopping point after blocking treatment.
- Assay suitable for downstream SDS-PAGE, western blot or mass spectrometry analysis.
Learn More
ML348

$60.00

Product code: A010-701

Highly potent, selective inhibitor of APT1 (Acyl Palmitoyl Thio-esterase 1). 14 fold greater Selectivity for APT1 over APT2 and other serine hydrolases.
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ML349

$60.00

Product code: A010-702

Highly potent, selective inhibitor of APT2 (Acyl Palmitoyl Thio-esterase 2).
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SiteCounter™ S-Palmitoylated Protein Kit

$499.00

Product code: K010-312
The new Badrilla SiteCounter^TM S-Palmitoylated protein kit is a quick and easy way to quantify the extent of S-palmitoylation of your protein of interest. Using a straightforward mass tag approach, modification of each cysteine residue results in an incremental increase in the mass of the protein. The number of mobility shifts (as detected by western blot analysis) is observed as a ladder of protein bands, each ‘step’ in the ladder representing an additional s-palmitoylated cysteine residue.

Kit highlights
- Enables determination of the number of S-palmitoylation sites on a protein
- Enables calculation of S-palmitoylation stoichiometry
- Kit designed to process 2 x 6 biological samples with paired negative controls (i.e. 24 samples in total).
- Convenient stopping point after blocking treatment.
- Assay suitable for downstream western blot analysis
Learn More
SiteCounter™ S-Palmitoylated Protein Mini Kit

$199.00

Product code: K010-313
The new Badrilla SiteCounter^TM S-Palmitoylated Protein Mini Kit is a quick and easy way to quantify S-palmitoylation. Competitively priced, this starter kit provides a gateway into determining the number of palmitoylation sites in your protein of interest

Kit highlights
- Competitively priced entry level kit.
- Enables detection of the number S-palmitoylated sites within your protein of interest and calculation of palmitoylation stoichiometry.
- Kit designed to process 2 x 2 biological samples with paired negative controls (i.e. 8 samples in total).
- Convenient stopping point after blocking treatment.
- Assay suitable for downstream western blot analysis
Learn More