The Badrilla CAPTUREome^TM S-Palmitoylated protein kit is a simple and easy way of determining whether your protein of interest is post translationally modified with a palmitate group. The assay is based on the acyl-RAC (resin assisted capture) method and facilitates, with four simple steps – Block, Cleave, Capture and Analysis -  determination of protein palmitoylation.

Kit highlights

• Enables capture of S-palmitoylated proteins.
• Kit designed to process 4 x 5 biological samples with paired negative controls (i.e. 40 samples in total).
• Convenient stopping point after blocking treatment.
• Assay suitable for downstream SDS-PAGE, western blot or mass spectrometry analysis.

The new CAPTUREome^TM S-Palmitoylation Mini Kit from Badrilla represents an affordable gateway into the emerging field of palmitoylation research. The Mini Kit, based on acyl RAC (resin assisted capture) technology, enables rapid determination of protein palmitoylation status from as little as 1mg of total cell lysate protein.

• Competitively priced entry level kit.
• Enables quick and easy capture of S-palmitoylated proteins from as little as 1mg of total cell lysate protein.
• Kit designed to process 2 x 3 biological samples with paired negative controls (i.e. 12 samples in total).
• Convenient stopping point after blocking treatment.
• Assay suitable for downstream SDS-PAGE, western blot or mass spectrometry analysis.

Badrilla’s Click S-Palmitoylation Detection⁷⁷⁵ Kit^TM provides a quick, convenient and robust method to identify S-palmitoylation of heterologously expressed proteins in cultured cells. The assay is based on the copper catalysed click chemistry reaction and facilitates, with four simple steps – Label, Lyse, Click and Detect - determination of protein palmitoylation.

Click S-Palmitoylation Detection⁷⁷⁵  Kit Enables

•           Detection of S-palmitoylated proteins in cultured cells.

•           Processing of 4 x 24 well cell culture plates (i.e. 96 samples in total).

•           Convenient stopping point after labelling treatment.

Highly potent, selective inhibitor of APT1 (Acyl Palmitoyl Thio-esterase 1). 14 fold greater Selectivity for APT1 over APT2 and other serine hydrolases.

Highly potent, selective inhibitor of APT2 (Acyl Palmitoyl Thio-esterase 2). 

The new Badrilla SiteCounter^TM S-Palmitoylated protein kit is a quick and easy way to quantify the extent of S-palmitoylation of your protein of interest. Using a straightforward mass tag approach, modification of each cysteine residue results in an incremental increase in the mass of the protein.  The number of mobility shifts (as detected by western blot analysis) is observed as a ladder of protein bands, each ‘step’ in the ladder representing an additional s-palmitoylated cysteine residue.

Kit highlights

• Enables determination of the number of S-palmitoylation sites on a protein
• Enables calculation of S-palmitoylation stoichiometry
• Kit designed to process 2 x 6 biological samples with paired negative controls (i.e. 24 samples in total).
• Convenient stopping point after blocking treatment.
• Assay suitable for downstream western blot analysis